Blind patch clamping was first done in vitro and only later performed in vivo 8, 9, 10. Recently, the patch clamp technique has been automated and improved to a more advanced level 6, 7. Therefore, there is a high demand to efficiently automate this labor intense and challenging process. The fundamental technique to achieve such trimodal characterization of neurons is the patch clamp recording, which is highly laborious and expertise intense. Recording morphological, electrophysiological, and transcriptional properties of neurons requires different techniques combined on the same sample such as patch clamp electrophysiology, posthoc morphological reconstruction, or single-cell transcriptomics. Revealing cell-type heterogeneity is still incomplete and challenging since classification based on quantitative features requires large amounts of individual cell samples, often thousands or more, encompassing a highly heterogeneous cell population. By characterizing molecular, morphological, connectional, physiological, and functional properties several neuronal subtypes have been defined 4, 5. It is well accepted now, that the complexity of the rodent and human cortex can be best resolved by classifying individual neurons into subsets by their cellular phenotypes 1, 2, 3. Research of the past decade uncovered the unprecedented cellular heterogeneity of the mammalian brain.
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